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KMID : 0545120100200091331
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 9 p.1331 ~ p.1338
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The Protective effects of Curcuma longa Linn. extract on carbon tetrachloride-induced hepatotoxicity in rats via upregulation of Nrf2
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Lee Hyeong-Seon
Li Li
Kim Hyun-Kyung
Dinesh Bilehal
Wei Li
Lee Dong-Seok
Kim Yong-Ho
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Abstract
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This study was designed to investigate the protective effect of Curcuma longa Linn extract (CLE) on carbon tetrachloride (CCl4)-induced hepatotoxic in rats. Male Sprague-Dawley rats were pretreated with 50mg/kg, 100mg/kg of CLE or 100mg/kg of butylated hydroxytoluene (BHT) for 14 days before CCl4 administration. In addition, CLE control group was pretreated CLE 100mg/kg only for 14 days. Three hours after the final treatment, a single dose of CCl4 (20 mg/kg) was administrated intraperitoneally (i.p.) to each group. After completion of the experiment, food and water was removed 12 hr prior to sacrifice. Rats were anesthetized by urethane and were collected blood and liver. The aspartate aminotransferase and alanine aminotransferase activities of serum and the hepatic malondialdehyde level significantly decreased in the CLE group than that in the CCl4-treated group. The antioxidant, such as superoxide dismutase, catalase, glutathione peroxidase activity and glutathione content increased considerable on the CLE group compared to the CCl4-treated group. The phase Ⅱ detoxifying enzyme, such as glutathione S-transferase, significantly increased in the CLE group than the CCl4-treated group. The content of Nrf2 was determined by western blot analysis. Pretreated CLE increased the level of Nrf2 to translocated in nuclear and Nrf2 increased activity of the antioxidant and phase Ⅱ detoxifying enzymes. These result indicated that CLE has protective effects against CCl4-induced hepatotoxicity in rats via activities of the antioxidant and phase Ⅱ detoxifying enzyme and through activation of Nrf2 to translocated in nuclear.
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KEYWORD
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Curcuma longa Linn extract, Carbon tetrachloride, Hepatotoxicity, Antioxidant, PhaseⅡ detoxifying enzyme, Nuclear factor-erythroid 2 (NF-E2)-related factor
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